nebnext microbiome dna enrichment kit (New England Biolabs)

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New England Biolabs nebnext microbiome dna enrichment kit
Nebnext Microbiome Dna Enrichment Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 359 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebnext+microbiome+dna+enrichment+kit/NEBNext+Microbiome+DNA+Enrichment+Box+1/pmc13055356-66-7-12
Average 97 stars, based on 359 article reviews

nebnext microbiome dna enrichment kit - by Bioz Stars, 2026-09

97/100 stars

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DNA Extraction:

Article Title: Host depletion kits improve microbiome analyses in environmental samples: Seagrass as a test case
Article Snippet: .. In addition, we tested a post-extraction host depleted method which included DNA extraction with the Wizard HMW DNA Extraction Kit (Promega, Cat. No. A2920), followed by the NEBNext Microbiome DNA Enrichment Kit (New England Biolabs, Cat. No. E2612S/L) to selectively remove eukaryotic CpG-methylated DNA via magnetic beads. ..

Magnetic Beads:

Lysis:

Article Title: Assessment of the effectiveness of host depletion techniques for profiling fish skin microbiomes and metagenomic analysis
Article Snippet: .. Popular DNA depletion kits and methods include the NEBNext Microbiome DNA Enrichment Kit (E2612L) from New England BioLabs, Molzym’s Molysis kits (D-321-050), Zymo’s HostZERO microbial DNA kit (D4310), and the selective lysis and degradation of eukaryotic cell DNA described by Hunter et al. ( ). .. In this study, we assess these three commercial kits (The NEBNext Microbiome DNA Enrichment Kit , the “ Molysis ” method, and the “ Zymo ” method—a shortened version of the HostZERO Microbial DNA Kit [D4310]) as well as the Hypotonic lysis method by Hunter et al. ( ) with modifications by Marotz et al. , and Nelson et al. ( ) and an alternative to the Hypotonic Lysis method employing phosphate-buffered saline (PBS) instead of deionized water.

Next-Generation Sequencing:

Article Title: Method for digesting nucleic acid in a sample
Article Snippet: .. A host DNA depletion of at least about 105, potentially resulting in a human:pathogen DNA ratio of 103:1, is required to facilitate NGS-based pathogen characterization in blood, a level of depletion (giving rise to pathogen nucleic acid enrichment) not achieved by methods disclosed in the art, such as commercially available pathogen DNA enrichment methods (QIAamp® DNA Microbiome Kit (Qiagen); NEBNext® Microbiome DNA Enrichment kit (NEB); MolYsis® Basic 5 kit (Molzym)). ..

Sequencing:

Article Title: Systemic campylobacteriosis in an American crow chick.
Article Snippet: .. The raw-extract DNA of the cerebrum was prepared for third-generation sequencing (NEBNext microbiome DNA enrichment kit; New England Biolabs). .. The DNA library was prepared (Ligation sequencing kit, SQK-LSK114; Oxford Nanopore Technologies) and sequenced (Flongle flow cell R10.4.1, Mk1B MinION device; Oxford Nanopore Technologies) for 24 h (Razer Blade 16, RTX4090 GPU; Razer).

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